A complete bacterial genome assembled de novo using only nanopore sequencing data
نویسندگان
چکیده
منابع مشابه
De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer.
Novel high-throughput DNA sequencing technologies allow researchers to characterize a bacterial genome during a single experiment and at a moderate cost. However, the increase in sequencing throughput that is allowed by using such platforms is obtained at the expense of individual sequence read length, which must be assembled into longer contigs to be exploitable. This study focuses on the Illu...
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Monitoring the progress of DNA through a pore has been postulated as a method for sequencing DNA for several decades 1,2. Recently, a nanopore instrument, the Oxford Nanopore MinION, has become available 3. Here we describe our sequencing of the S. cerevisiae genome. We describe software developed to make use of these data as existing packages were incapable of assembling long reads at such hig...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2015
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.3444